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·Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Yrbio offer the E. coli expression services.
Procedure
1. ·Foreign gene design and codon optimizing.
2. Reading frame design and/or cds synthesis.
3. Plasmid constructing.
4. Plasmid transformation and expression strain selection.
5. Incubation and induction of protein expression.
6. Protein purification.
7. Protein verification.
Service Quote and Delivery Time:
| Service Contents | Price (￥) | Delivery Time |
|---|---|---|
| Gene Synthesis (Optional) | 4￥/bp | 2-3 Weeks |
| Plasmid Constructing (Optional) | 1000 | 1-2 Weeks |
| LacZ & trpE Recombinant protein expression and detection | 3000 | 2-3 Weeks |
| GST-recombinant protein expression and detection | 3000 | 2-3 Weeks |
| Thioredoxin recombinant protein expression and detection | 3000 | 2-3 Weeks |
| Custom protein expression | 3000 (Inquiry) | 2-3 Weeks |
| Large Scaled Protein Expression | Inquiry | 4-6 Weeks |
1. Yrbio will finish the protein expression experiments ASAP and offers the qualified proteins to customers.
2. Yrbio provide the detailed report (include procedure, instruments, E. coli strains, yield conditions, and raw data and images)
1. Customer should ensures that the materials provided are correct (including gene templates, primers, expression vectors).
2. Customer is AOR for the mutated gene templates or expression vectors.
| Address: | Building 1, M3 group, No. 315, Yinpeng South Rd, ChangSha City, Hunan Province, China PRC. |
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| Tel: | 0731-84318757（Project Dept）/84314166（Market）/84317068（Tech Services） | ||
| Tech Support: |
(+86)132-7202-1968 (Dr. Xiao) |
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| Fax: | (+86)-731-8431-8757 | ||
| Email: |
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