Promoter Obtaining-->Plasmid Constructing--> Tranfect to the host cells-->Fluoresence collecting-->Data processing

   Reporter genes have become an invaluable tool in studies of gene expression. They are widely used in biomedical and pharmaceutical research and also in molecular biology and biochemistry. A gene consists of two functional parts: One is a DNA-sequence that gives the information about the protein that is produced (coding region). The other part is a specific DNA-sequence linked to the coding region; it regulates the transcription of the gene (promoter). The promoter is either activating or suppressing the expression of the gene. The purpose of the reporter gene assay is to measure the regulatory potential of an unknown DNA-sequence. This can be done by linking a promoter sequence to an easily detectable reporter gene such as that encoding for the firefly luciferase. Common reporter genes are β-galactosidase, β-glucuronidase and luciferase. Various detection methods (see below) are used to measure expressed reporter gene protein. These include luminescence, absorbance and fluorescence. The advantages of a luciferase assay are the high sensitivity, the absence of luciferase activity inside most of the cell types, the wide dynamic range, rapidity and low costs. The Promega's Dual-Luciferase™ Reporter (DLR) Assay System contains two different luciferase reporter enzymes that are expressed simultaneously in each cell. The firefly luciferase and the Renilla (sea pansy) luciferase can discriminate between their respective bioluminescence substrates and do not cross-activate. Yrbio offer the dual-luciferase services for the customers.

Customer Notices

1. For the Dual Report assay, customer should provide the Promega Dual-Luciferase™ Assay Kit (Yrbio also provides the modified reporting vectors).
2. Customer provide the promoter or purchase from Yrbio promoter bank.
3. Customer provide the detailed information about the promoter, Yrbio will discuss with customer to integrate suitable fragments to the report vectors.
4. Customer assign the host cells and provide the drug treatment protocols.
5. Yrbio provide the fluorescence collecting service.

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