Methylation Specific PCR (MSP) is a bisulfite conversion based PCR technique for the study of DNA CpG island methylation. For MSP experiment, two pairs of primers are needed (one pair specific for methylated DNA (M) and the other for unmethylated DNA (U)). To detect methylated or unmethylated DNA, in each primer (or at least one of the pair) sequence, one or more CpG sites are included. First, DNA is modified with sodium bisulfite and purified. Then, two PCR reactions are performed using M primer pair and U primer pair. Successful amplification from M pair and U pair indicated methylation and unmethylation respectively. Please check out training centre for the MSP protocols.

Service Decription

  MSP is a high sensitivily assay and is convenient to test large scale samples.

1. Yrbio offers genome DNA purification services.
2. MSP primer design is free of charge.
3. For primer synthesis, please check out Yrbio primer services.
4. Data processing is free of charge.

Delivery Time

Customer Notices

• 1. Customer should provide the purified genome DNA with detailed informations (agarose gel images, concentrations, solutions), or provide the fresh samples for genome DNA extraction.
• 2.  Yrbio will purify the DNA with DNA purification kits.
• 3.  Yrbio will discuss with the customer on the selection of target CpG island,  however, Yrbio DO NOT guaratee the anticipated results.
• 4.  For the special DNA structure, customer should be beware of the failure risks.

Delivery Packages

• 1.  Purified DNA samples.
• 2.  Primer sequences and related informations.
• 3.   MSP procedure and results, including the agarose gel images.