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RNAi外源筛靶上市!

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2011武汉国际生物技术展

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PCR/ Sequencing for Gene Mutation Analysis

The DNA sequencing and mutation analysis use a ABI automated DNA sequencer and a routinely PCR machine. The use of dHPLC is to study polymorphisms and in mutation screening of genes associated with cancer. A four capillary DNA sequencer takes care of the DNA sequencing needs of scientists on campus as well as from other institutes. Using of MSP method to detect methylation of DNA sequences. The average delivery time is two working days after receiving samples. Users of DNA sequencing facility are charged minimally per sequencing reaction. Recently the sequencing facility has been used for sequence analysis of genes involved in cell cycle dysfunction, tumorigenesis and cancer predisposition as well as to confirm the engineered mutations in cloned inserts

Service Contents

The sequencing for gene mutation analysis includes:

  1. Sequencing
  2. Nested PCR/Sequencing
  3. Nested PCR/Sequencing
  4. Mutant enriched PCR/Sequencing

Apply Fields of Mutation Sequencing

  1. Researches in the mechanism of tumorigenesis.
  2. Predict the efficiency of tumor gene related drugs.
  3. Stable cell line screen.
  4. Tumor malignization prediction.

Service Description

  1. Using a broad range of samples. 
  2. Using high fidelity PCR which can amplify the low abundance transcripts.
  3. We can screen the appointed gene mutation (including EGFR, K-RAS, PI3K, p53, p16, BRCA1, BRCA2).
  4. Our experts will use the professional software to analyse the sequencing map. Without omitting any heterozygous mutations.

Customer Notices

  1. Customer should provide the detailed informations about the research interests.
  2. We accept broad range of samples, including genomes, cells, biopsy samples and blood.
  3. The analysis result about the sequencing data, including the mutation type, positions, and the aa mutations.

Delivery Data

  1. Sequencing map & results.
  2. Mutation analysis data.
  3. Experiment reports.

Service Quote

Inquiry

Contacts

Address: Building 1, M3 group, No. 315, Yinpeng South Rd, ChangSha City, Hunan Province,  China PRC.
Tel: 0731-84318757(Project Dept)/84314166(Market)/84317068(Tech Services)
Tech Support:
(+86)132-7202-1968 (Dr. Xiao)
Fax: (+86)-731-8431-8757
Email: