Beside the Yeast-Two-Hybrization assays, Yrbio offer GST/His-pull dowm and Immunoprecipitation assays to verify the protein-protein interactions.

GST/His-pull Down

   The pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. Pull-down assays are useful for both confirming the existence of a protein-protein interaction predicted by other research techniques (e.g., co-immunoprecipitation) and as an initial screening assay for identifying previously unknown protein-protein interactions. Pull-down assays are a form of affinity purification and are very similar to immunoprecipitation, except that a "bait" protein is used instead of an antibody.

Co-IP Assay

  Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure. Immunoprecipitation of intact protein complexes (i.e.: antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). Co-IP works by selecting an antibody that targets a known protein that is believed to be a member of a larger complex of proteins. By targeting this known member with an antibody it may become possible to pull the entire protein complex out of solution and thereby identify unknown members of the complex.

Delivery Time

Customer Notices

1. Yrbio offer GST/His recombinant protein expression and continues the pull down experiments.
2. Customer should provide excellent antibodies (especially for co-IP assay) or purchase from Yrbio.
3. Customer design experiments, if the Pull Down/Co-IP assay come out negative results, Yrbio will repeat the experiment without extra charges.
4. Yrbio offer the detailed reports (including procedure, instruments, raw data and figures, and the light chain bands of the negative results).

See Also qPCR protein-protein interaction assay