RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA. Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction.

    Obtaining high quality, intact RNA is the first and often the most critical step in performing many fundamental molecular biology experiments, including Northern analysis, nuclease protection assays, RT-PCR, RNA mapping, in vitro translation and cDNA library construction. To be successful, however, the RNA isolation procedure should include some important steps both before and after the actual RNA purification. Yrbio have ten years experience in RNA extraction, and offers RNA extraction kit.

Service Contents

  Obtain whole RNAs from cells/tissue of animals/plants/bateria/yeast.

Procedure

1. Pretreatments

 Customer should provide fresh or cryopreserved newly made samples, avoid repeat freeze.

Plant Tissues—— Liquid nitrogen grinding. 
Animal Tissues——Homogenate/Liquid nitrogen grinding
Cultured Cells—— Protein Kinase K  (PK) digestion
Bacteria——lysozyme digestion
Yeast——Liquid nitrogen grinding/Break with glass beads

2. Cell Lysis
3. RNA Purification
4. RNA verification
5. RNA Purity Detection

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