Reverse transcription polymerase chain reaction (RT-PCR) is a variant of polymerase chain reaction (PCR), a laboratory technique commonly used in molecular biology to generate many copies of a DNA sequence, a process termed "amplification". In RT-PCR, however, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme reverse transcriptase, and the resulting cDNA is amplified using traditional or real-time PCR. Yrbio follow the rules of MIQE to prepare the cDNA samples for qPCR.

Service Contents

• 1.  RT-PCR test the gene expression abundance. 
• 2.  Test the transcription of shRNAs.
• 3.  RT-PCR to test the gene expression viabilities during treatments, using β-Actin or GAPDH as control.
• 4.  Prepare cDNAs for qPCR assay. 

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RNA Extraction( whole RNA extraction)

RT-PCR

PCR