Yrbio shRNA constructs were designed using siRNA algorithm considering sequence, specificity and position scoring for optimal hairpins. The hairpin consists of a 21-base stem and a 6-base loop. The hairpins were cloned into the eukaryotic expression vector and sequencing verified. Yrbio possess more than 10,000 human and mouse gene, over 50,000 shRNA libraries. Experiment verified that more 90% of the shRNA are effective and the overall silencing efficiency reaches 90%. To silence the target gene, Yrbio will provide 3~5 shRNA construct consiting the siRNA sequences. Pricing 3800￥/Gene(3~5 shRNA constructs). Yrbio also provide lentiviral packing services for the shRNA silencing. 

 

Figure 1 shRNA Vecotr Map (pYrbio-LT-1 Vector)

Severice Quote

Provide a suite of  shRNA  constructs (includes 3~5 shRNA constructs) for a target gene,  3800￥, Delivery time: 3~5 weeks.

Service Commitments

  Yrbio promised that at least one construct in the shRNA suite is effective, otherwise Yrbio will full refund.