Service Desription

   Yrbio will reports to the customer on selecting the possible colony candidates. For stable cell lines, Yrbio is experienced on Tet-ON controllable expression system.

Service Commitments

1. Customer provide the plasmid, detailed informations about the foreign genes, the host cells, PCR primers and antibodies as needed.
2. Yrbio following the information provided to construct the plasmid and Maxi-prep the plasmid for transfection. 
3. Yrbio can provide the host cell (please check out Yrbio cell repository for the host cells), screen drugs (optional).
4. Yrbio provide the RT-PCR and Western-blot testing.
5. Yrbio provides the cryopreserved stable cell line and the construct plasmid (also a glycerin baterial containing the plasmid).
6. Yrbio provide the detailed report, including the experimental procedure, protocols, instruments, and raw data and images. 

Procedure

• 1. Plasmid constructing and Max-prep of plasmids, passage cell for transfection at the same time; 
• 2. Transient transfection of  host cells and screen for suitable concentration of the cells;
• 3. Screen the cells by drugs, pick up colonies (or screen for monocolonies using dilution method); 
• 4. Preliminary test the antibodies to test the expression of the foreign genes (companion with step 3); 
• 5. Western-blot  and/or RT-PCR testing; 
• 6. Turn over the cryopreserved stable cell lines and the detail reports/results.

Service Quote