RNAi外源筛靶上市!

2011武汉国际生物技术展

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Yeast Expression Platform

   Yeast is a eukaryotic organism and has some advantages and disadvantages for protein expression as compared to E. coli. One of the major advantages is that yeast cultures can be grown to very high densities, which makes them especially useful for the production of isotope labeled proteins. These single-celled eukaryotic organisms grow quickly in defined medium, are easier and less expensive to work with than insect or mammalian cells, and are easily adapted to fermentation. Yeast expression systems are ideally suited for large-scale production of recombinant eukaryotic proteins. Yrbio's yeast expression platform provides a flexible, controllable, and effective solution for protein expression needs. Our services includes bioinformatics analysis, domain selection, vector design, tag selection, sequence confirmation, cell transfection, cell amplification, purification by tag selection, yield optimization, scale up processing, and protein characterization.。

Procedure

  • 1. Plasmid Constructing: Subcloned the foreign genes into at least two expression vectors, maxi-prep of plasmids.
  • 2.  Optimizing Yield Conditions:  Transform the linear plasmids into the host yeasts, optimizing the yield conditions (followed by SDS-PAGE and Western Blot detection.
  • 3.  Protein Purification and Characteriation.

Delivery Time

Contents Price (¥)  Delivery Time
Gene Synthesis (Optional) 4¥/bp 2~3 Weeks
Plasmid Construction (Optional) 1000 1~2 Weeks
Optimizing Yield  Conditions and SDS-PAGE Verification 5000 2~3 Weeks
Western Blot detection (Optional) 1000 1 Week
Large Scale Protein Expression (Optional) Inquiry 2~3 Weeks

Customer Notices

  • 1. Customer should ensures that the materials provided are correct (including gene templates, primers, expression vectors).
  • 2. Customer is AOR for the mutated gene templates or expression vectors.

Contacts

Address: Building 1, M3 group, No. 315, Yinpeng South Rd, ChangSha City, Hunan Province,  China PRC.
Tel: 0731-84318757(Project Dept)/84314166(Market)/84317068(Tech Services)
Tech Support:
(+86)132-7202-1968 (Dr. Xiao)
Fax: (+86)-731-8431-8757
Email: